Abstract: A hydrocarbon fluid storage structure has a storage vessel containing hydrocarbon fluids therein and a containment vessel fully surrounding the storage vessel so as to provide secondary containment to the storage vessel. A heater is surrounded by heat exchanger fluid within a heat exchanger vessel within the storage vessel. The exterior of the containment vessel is insulated. The heat exchanger fluid is passively circulated within a closed loop path defined by the heat exchanger vessel. Connecting valves providing external access to the contents of the storage vessel are received within a valve housing in heat exchanging relation with a containment space between the containment vessel and the storage vessel.

Abstract: The present invention relates to a liquid composition comprising living green microalgae for improving plant growth. The liquid composition is prepared by growing green microalgae at a pH of 10-12 in the presence of hydrogen carbonate as the inorganic carbon source. The liquid composition may be stored at 4 to 25 degrees C. until use. The liquid composition may be used to improve fertilizers.

Abstract: An algae cultivation system may include: a plurality of panels within a cultivation container, positioned along a first axis perpendicular to the gravitational force, wherein a cultivation volume is created between each pair of panels, and wherein the cultivation volumes are fluidly coupled so as to allow horizontal flow therebetween along the first axis; at least one first sparger, to distribute a first fluid into the container at a first operating flow rate; at least one second sparger, to distribute a second fluid into the container at a second operating flow rate; and at least one controller, to control the first operating flow rate and the second operating flow rate. The first operating flow rate may be adapted to allow turbulent mixing the algae in the cultivation container, and the second operating flow rate may be adapted to allow assimilation of materials in a liquid in the cultivation container.

Abstract: Provided is a method of culturing one or more microorganisms. The method includes culturing one or more microorganisms in a medium comprising crude glycerol at a first concentration level, feeding to the media an additional amount of crude glycerol, once the first concentration of glycerol is reduced to a first threshold level, at a concentration sufficient to achieve the first concentration level, monitoring the crude glycerol concentration until the first concentration level of the crude glycerol is reduced to the first threshold level. The steps may be repeated until a desired microorganism cell density is achieved.

Abstract: The invention discloses a fermentation method for production of fucoxanthin by Nitzschia laevis, including the following steps of: step A, preparation of inocula; step B, fermentation culture: inoculating of Nitzschia laevis according to a certain volume ratio to reaction kettle containing sterile fermentation medium for aeration fermentation, preparing fucoxanthin fermentation broth through culture mean of fed-batch nutrient components; step C, obtaining high fucoxanthin induction culture solution by aeration induction culture under irradiation of monochromatic light or mixed light; extracting fucoxanthin from high fucoxanthin induction culture solution.

Abstract: The present invention relates to a particulate material separation assembly. It comprises a filtration membrane and an antifouling device. The antifouling device comprises one or more magnets and a plurality of magnetisable particles. The one or more magnets cause the plurality of magnetisable particles to self-assemble into dynamic bristles, thereby forming a brush. The particulate material separation assembly is particularly useful in the context of micro algae harvesting.

Abstract: A method and system for reducing the concentration of nitrogen-bound nitrate from at least one of groundwater, surface water, or waste water is disclosed. The system includes an algal photobioreactor system. The photobioreactor includes a transparent tubular structure, a medium disposed inside the transparent tubular structure, and alga configured to adhere to the medium. The photobioreactor further includes a pump, and a light source. The method includes providing contaminant-containing water, adding the water to the algal photobioreactor system, adjusting at least one of temperature, ion concentration, CO2 concentration, pH, light wavelength, ion concentration or light intensity in the algal photobioreactor system to enhance growth of algae, removing water from the algal photobioreactor system, and harvesting the algal biomass. A system and method are also disclosed for reducing the concentration of organophosphates from water via an organophosphate-removing alga.

Abstract: The present invention relates to a large-scale photosynthetic bioreactor in which transparent photobioreactors including a baffle are connected in parallel or in series by an adhesive element so that the reactor volume can be easily scaled up for scale-up culture of photosynthetic organisms, and to a fabrication method thereof. The large-scale photosynthetic bioreactor according to the present invention makes it possible to culture a larger amount of microalgae than a conventional photobioreactor in the same area. In addition, it has high light transmittance, produces a large amount of biomass per unit area due to smooth mixing, and has a significant effect of reducing carbon dioxide. Furthermore, the present invention has an advantage in that the number of photosynthetic bioreactors required to culture the same scale of photosynthetic organisms is significantly smaller than that in a conventional process for culture of photosynthetic organisms, and thus the operating costs can be reduced.

Abstract: The present disclosure relate to feed ingredients and formulated feed, methods for their manufacture, and uses thereof in nutritional applications such as in aquaculture, terrestrial animal feed, and human nutrition. The feed ingredient compositions comprise lysed microbial cells with a small aspect ratio and triglyceride oil.

Abstract: A device (1) for accommodating a disposable container (44) has a receptacle (10) with at least one receptacle wall (16) that defines an interior of the receptacle (10) for accommodating the disposable container (44). The device includes a temperature control unit for controlling the temperature of the receptacle wall (16). A lighting device (50) is located on an inner face of the receptacle wall (16) that faces the interior of the receptacle (10) and that has the temperature controlled by the temperature control unit. The lighting device (50) is designed to emit light into the interior of the receptacle (10).

Abstract: The present application belongs to the technical field of biology. Provided is a photobioreactor used for algae cultivation, said photobioreactor comprising: a reactor main body, a separation unit, and a first aeration device. The reactor main body is a sealed irregular tubular shape, the separation unit is located within the reactor main body, and divides the reactor main body into two spaces, a left space and a right space, and the first aeration device is connected to a bottom portion of the reactor main body. Also provided is an algae cultivation system, comprising the photobioreactor, the second aeration device, and a temperature control system, and being capable of regulating the temperature of an algae solution.

Abstract: A method of producing the constituents of a therapeutic product from mammalian cells is described herein. Cells are isolated from a mammalian source. The cells are exposed to supercritical carbon dioxide (SCCO2) for 1 to 30 minutes, where the SCCO2 is maintained at a pressure of 1071 pounds per square inch (PSI) and a temperature of 31.1 to 45 degrees Celsius during the exposure. The exposure dissociates the cellular membranes of the cells to release intramembrane components therein to produce constituents of the therapeutic product. The mammalian cells may include at least one of platelets, stem cells, germ cells, and somatic cells. The methods described herein are particularly advantageous for releasing and capturing therapeutic intramembrane components from platelets and alpha-granules.

Abstract: An apparatus (10) for lifting and moving an object that is underwater. The apparatus including a first (12) and a second (14) chamber, both chambers containing a first (16) and a second (18) fluid. There is a conduit (20, 21) in fluid communication with the first and second chambers, the first and second fluids moveable between the first and second chambers.

Abstract: The process involves extracting carboxylic acids having from one to nine carbons, the carboxylic acids produced by microorganisms in a fermentation reactor by anaerobic fermentation from fermentable biomass. The extraction is a liquid-liquid type, and includes a) choosing an endogenous extraction solvent among at least one of the carboxylic acids, such that the number of carbons of the solvent is greater than or equal to the number of carbons of the carboxylic acid to be extracted, b) bringing the extraction solvent chosen into contact with the fermentation medium, without interrupting the fermentation, outside the fermentation reactor, c) separating (6) the fermentative metabolites from the extraction solvent by at least one distillation, and d) collecting and storing or using the fermentative metabolites obtained in step c). The invention also relates to a facility for carrying out the process.

Abstract: The invention provides for the integration of a gas fermentation process with a gasification process whereby effluent downstream from the gas fermentation process is recycled to the gasification process. The invention is capable of recycling one or more effluents including biogas generated from a wastewater treatment process, tail-gas generated from the fermentation process, unused syngas generated by the gasification process, microbial biomass generated from the fermentation process, microbial biomass generated from a wastewater treatment process, crude ethanol from the product recovery process, fusel oil from the product recovery process, microbial biomass depleted water, wastewater generated from the fermentation process, and clarified water from a wastewater treatment process to a gasification process.

Abstract: A method of binding a target metal in solution. The method of binding a target metal comprises contacting a solution containing i) a target metal with ii) an encapsulated exudate of a culture of algal flagellate, or a fraction thereof; or an encapsulated dried Euglena biomass or a fraction thereof, to form a complex between the target metal, and the encapsulated exudate or fraction thereof, or the encapsulated dried Euglena biomass or the fraction thereof; and optionally separating the complex from the solution. The disclosure also relates to a biosorbent element, as well as methods of using same in binding a metal in solution.

Abstract: A system for growing and reproducing microorganisms that includes a basin system that includes a number of basins, where each basin has a vertical meandering system which is formed by partitions and which can be illuminated, each basin is filled with a nutrient suspension, at least one outer wall of each basin is double-walled such that a cavity is formed, a temperature control medium for controlling the temperature of the nutrient suspension can flow through the cavity.

Abstract: A photobioreactor with a microalgae's cultivation chamber in the form of a duct that is constructed from an inflatable sleeve from transparent polymer film; this inflatable sleeve excluding its terminal sections is sandwiched between a bank of frames with wire nettings from below and glass panes from above the inflatable sleeve, and it has a small inclination regarding the horizontal plane. The terminal sections of the inflatable sleeve are in fluid communication with two headers, which are used for supplying and removal of gaseous medium and suspension of microalgae into and out of the duct.

Abstract: The invention relates to a bioreactor comprising a tank containing a culture medium in which a cell culture composed of algae cells is dispersed. The cell culture of algae has a concentration greater than 0.1 g/L in the culture medium, and each algal cell has a minimum absorption in a specific range of wavelengths of light. The bioreactor comprises a light source that is capable of emitting incident light in the direction of the tank, 60% of the photons of the incident light having a wavelength which is included in said specific range of wavelengths of light. The invention also relates to the use of the bioreactor for the production of biomass.

Abstract: It is an object of the present invention to provide the eukaryotic microalgae, which have been genetically modified such that larger amounts of assimilation products produced by photosynthesis are directed to the synthesis of triglyceride (=triacylglycerol; TAG), and specifically, the present invention relates to a genetically modified strain of eukaryotic microalgae, in which a gene encoding an AGL1 protein is highly expressed, or a gene encoding an FAT1 protein and/or a gene encoding a DGAT2 protein are further highly expressed, as well as the gene encoding an AGL1 protein, wherein TAG productivity is improved in comparison to the parent strain thereof.

Abstract: A process of growing a culture of cyanobacteria or algae using chitin or chitosan as a source of nitrogen for photosynthetic growth is described. This process can be used to remove pollutants from nitrogen-deficient natural waters or wastewaters including buchu mercaptan production wastewater. Biomass that results from photosynthetic growth on chitin can be used, either as whole cells or the isolated components of the cells, for a large variety of commercial purposes.

Abstract: The invention relates to the field of mass cultivation of microalgae and specifically relates to a horizontal submerged cover type carbon supplement device and carbon supplement method for cultivating microalgae in an open pond. The horizontal submerged cover type carbon supplement device for cultivating microalgae in an open pond, comprises: one or more than two covers and gas distributors, wherein the gas distributor is arranged at the bottom of the open pond and the cover is arranged above the gas distributor; the cover, in form of a cylinder with openings at both ends, is arranged along the flow direction of the culture solution, the two ends of the cover being a culture solution inlet and a culture solution outlet respectively; the gas distributor is kept away from the culture solution outlet of the cover by more than 40 cm.

Abstract: A method for processing algae-based products using flue gas heat includes mixing flue gas with water, receiving a heated gas in a first enclosure of a heat exchanger, receiving an algal paste in a second enclosure of the heat exchanger, introducing an organic solvent to the algal paste, extracting an algal oil and a plurality of algal shells from the algal paste by dissipating heat from the first enclosure to the second enclosure; and extracting the algal oil from the organic solvent and the algal shells.

Abstract: The present disclosure relates to a method for increasing lipid content in microorganisms. The method comprises decreasing the expression of molecules involved in the protein synthesis to decrease protein synthesis and thereby increase lipid synthesis in the microorganisms. The present disclosure also provides a modified microorganism having increased lipid content.

Abstract: Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided.

Abstract: Methods are provided to control the harvesting of desired metabolic byproducts from hypersaline photosynthetic algae. Methods are presented to control secretion of said metabolic products in monocultures and co-cultures after accumulation of byproducts. The metabolic byproduct secretion is controlled by controlling caspase activity.

Abstract: The purpose of the present invention is to effectively accumulate triacylglycerol in algae cells, the present invention providing a method for introducing a triacylglycerol synthetase gene, a phosphorus starvation-inducible promoter, and a 3? untranslated region into algae.

Abstract: Bioreactors and methods for cultivating microalgae are provided herein. The bioreactor and methods include features and modifications to improve heterotrophic growth efficiency by providing a light signal.

Abstract: The present invention discloses a selenium-doped nano-silica sol capable of both inhibiting the absorption and accumulation of heavy metal in rice and producing a selenium-rich rice, and a preparation method thereof. The selenium-doped nano-silica sol of the present invention is prepared mainly by using a nano-silica sol as a carrier, sodium selenite and the like as a raw material, vitamin C and the like as a reducing agent, and polyvinylpyrrolidone and the like as an emulsifier, and doping and dispersing selenium in a specific ratio in a silica sol so as to form a selenium-doped nano-silica sol with a special structure and function. The selenium-doped nano-silica sol prepared according to the invention has high stability and high concentration, and is uniform and transparent, and has a silica content of up to 20% or more, a selenium content of up to 1% or more, and low impurity content.

Abstract: The present invention relates to the continuous process of sequestration of nutrients from waste effluents released from gas fermentation plants or green-house gases emission managing plants by novel Thraustochytrids. Particularly this invention relates to the methods and systems to enhance sequestration rate and productivity of the process. This invention also relates to rapid biotransformation of nutrients present in waste effluents into high value omega-3 fatty acids like Docosahexaenoic acid (DHA), Docosapentaenoic acid (DPA), Eicosapentaenoic acid (EPA) and lipids for biodiesel. This disclosure is about means of processing of waste streams and producing value added products out of it.

Abstract: The present application is generally directed to separation and recovery of rare earths using biomass, liposomes, and/or other materials. In some embodiments, a composition comprising rare earths is exposed to biomass, where some of the rare earths are transferred to the biomass, e.g., via absorption. The composition may then be separated from the biomass. A solution may be exposed to the biomass thereby enriching the solution in one or more rare earths, relative to other rare earths in the biomass. The solution and the biomass may then be separated, and the rare earths recovered from the solution. In some cases, this process may be repeated with different solutions, which may result in different solutions enriched in various rare earths. Similar processes may be used to separate the rare earths from thorium and uranium. Liposomes may be used instead of and/or in addition to biomass.

Abstract: An object of the present invention is to construct a production system that enables efficient production of organic acids using blue-green algae, which are photosynthetic microorganisms, by utilizing carbon dioxide and thereby increasing an amount of organic acids produced. The present invention relates to blue-green algae overexpressing a clock protein gene and a method for producing organic acids by culturing the blue-green algae.

Abstract: A waste processing system includes: an input module for converting input organic waste into processed input waste; a digester module, coupled to the input module, for generating biogas and digester effluent from the processed input waste, including: a digester tank; baffles affixed within the digester tank; a digester process solution in the digester tank including, hydrolytic bacteria, acidogenic bacteria, acetogenic bacteria, methanogenic bacteria, or a combination thereof, for converting the processed input waste into the biogas and the digester effluent; and an output module, coupled to the digester module, for collecting the biogas and digester effluent after a residence period.

Abstract: The present invention relates to a method for the recovery of intracellular components of materials of a biological origin, such as yeasts, algae, bacteria and/or mildews, after fermentation/cultivation, which comprises a thermal lysing treatment via nebulization and the use of a nebulization solvent capable of extracting said intracellular components once the nebulization has been completed.

Abstract: Methods are disclosed for cultivation of oleaginous microorganisms on feedstocks including depolymerized cellulosic material such as corn stover, Miscanthus, forage sorghum, sugar beet pulp and sugar cane bagasse. Also disclosed are methods of manufacturing novel triglyceride oil compositions using cellulosic feedstocks, including oil compositions comprising fatty acids such as those having chain lengths of 8, 10, 12, and 14 carbon atoms.

Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide and salt are described herein. The method comprises dosing the culture comprising a concentration of salt with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises detecting the contamination and then dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Abstract: The present invention provides a cost effective biotechnological process for production of bio-fuels from isolated and characterized microalgae. The algal strains used in the present invention having higher biomass, higher lipid productivity, higher pH and temperature tolerance are selected from the group consisting of Chlorella vulgaris iOC-1, Chlorella vulgaris iOC-2, Chlorella kessleri, Botrococcus bruni, Dunaliella salina and Nannochloris oculat or a combination thereof having 95-100% similarity with 18s ribosomal nucleic acids nucleotide sequences (rDNA) given for Seq. ID I, Seq. ID 2, Seq. ID 3, Seq. ID 4, Seq. ID 5 and Seq. ID 6. The present process of bio-fuel production comprises the steps of producing lipid from green algae in bioreactors by various novel steps and extracting oil from dried algal cells and ultimately producing biodiesel by transesterification of the said extracted oil.

Abstract: The present invention comprises methods and compositions comprising a microalgae consortium. A composition comprises at least one genera of microalgae flocculated by Paenibacillus polymyxa strain, Strain 2, deposited under the Budapest Treaty as ATCC Accession No. PTA-12841. Methods of treating the soil comprise adding a composition of the present invention to soil.

Abstract: A method of damaging cell structure of an aquatic substance includes: providing an aquatic substance raw material, where the aquatic substance raw material includes an aquatic substance; adjusting a water content in the aquatic substance raw material to form an aquatic substance slurry to be processed; placing the aquatic substance slurry to be processed in a pressure container; introducing a compressed gas into the pressure container to enable the compressed gas and the water in the aquatic substance slurry to be processed to form an acidic fluid, and making the cell structure of the aquatic substance hydrolyzed and damaged by the acidic fluid; and performing a depressurizing step to separate the compressed gas.

Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with salt are described herein. The method comprises dosing the culture comprising with a concentration of salt based on the stage of the cells in the culturing process to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Abstract: Methods of treating lysis in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises detecting lysis in the culture and then dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Abstract: The present invention relates to a method for photoculturing and harvesting microalgae, and more particularly to a method for photoculturing and harvesting microalgae, in which a light emitting diode (LED) is used as a light source to increase the efficiency with which microalgae are cultured, and the cultured microalgae are harvested using a natural polymer flocculant and air microbubbles so that the operating cost is reduced and the range of application of microalgae is expanded, and also a semi-continuous culture method is applied so that the maximum concentration of microalgae can be produced again within a short time, even though the amount of microalgae harvested is small.

Abstract: A method and system for processing algae involves the use of an ionic liquid-containing clarified cell lysate to lyse algae cells. The resulting crude cell lysate may be clarified and subsequently used to lyse algae cells. The process may be repeated a number of times before a clarified lysate is separated into lipid and aqueous phases for further processing and/or purification of desired products.

Abstract: The invention relates to a method for cultivating photoautotrophic micro-organisms, comprising the following steps: provision of a photosynthesis layer, which contains a photosynthesis medium and photoautotrophic micro-organisms suspended therein, in a closed accommodation space having a gas-diffusion-inhibiting outer boundary, wherein the photosynthesis layer has an average thickness of no more than 10 mm; exposure of the photosynthesis layer without eddying or feeding through a gas in the photosynthesis medium while limiting the diffusive gas exchange of the accommodation space by the environment, wherein the supply of micro-organisms in the photosynthesis medium with hydrogen carbonate ions is maintained by storing hydrogen carbonate ions in the photosynthesis medium and/or providing a photosynthesis substrate in the form of carbon dioxide and hydrogen carbonate from a cell-free carbon storage medium outside the photosynthesis layer; and conducting a gas formed during photosynthesis out of the accommodatio

Abstract: A method and system are described for on-board treatment of an exhaust stream containing CO2 emitted by a hydrocarbon-fueled internal combustion engine (ICE) used to power a vehicle in order to reduce the amount of CO2 discharged into the atmosphere which include: a.

Abstract: The present invention is an oral dosage composition for prevention and treatment of hepatic inflammation such as NASH. The present invention is an oral dosage composition for prevention or treatment of hepatic inflammation, contains the radical scavenging active spirulina powder effective in preventing, ameliorating, and curing NASH or the like.

Abstract: The present invention provides bicarbonate containing and/or bicarbonate-producing compositions and methods to induce lipid accumulation in an algae growth system, wherein the algae growth system is under light-dark cycling condition. By adding said compositions at a specific growth stage, said methods lead to much higher lipid accumulation and/or significantly reduced total time required for accumulating lipid in the algae growth system.

Abstract: The invention relates to a process for the production of algal biomass with a high lipid content, comprising: (a) the production of inocula in order to effect phase (b), in photoreactors; (b) the massive cultivation of the algal biomass in open ponds, inoculated with phase (a); (c) a thickening phase of the algal biomass, effected blandly; (d) an induction phase of the lipid production, wherein modules are used consisting of photoreactors or open ponds; (e) a separation phase of the biomass with a high lipid content.

Abstract: Provided is a method of culturing green algae which promotes the growth of the green algae which is in a state of being a green swarm cell by irradiating the green algae that accumulate astaxanthin with an artificial light, in which the green algae are grown in a liquid medium while maintaining a state in which the color of a culture solution of the green algae is green or brown by intermittently radiating a blue illumination light while continuously radiating a red illumination light.